Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The HIV-1 HLA-A2-SLYNTVATL is a help-independent CTL epitope.

doi: 10.4049/jimmunol.172.9.5249

Figure Lengend Snippet: FIGURE 1. Different requirements for ex- ogenous cytokines for the generation of SL9- and IV9-CTLs. A and B, Cytotoxicity was de- termined on day 30 at an E:T ratio of 5:1 by the chromium release assay. All cultures were gen- erated in the presence of IL-7 (10 ng/ml). IL-2 (20 U/ml) and/or IL-10 (10 ng/ml) were added to the cultures indicated after first restimulation. C, Increasing specific cytotoxicity of the SL9- CTLs expanded with only IL-7 and in the ab- sence of the other cytokines over a 6-wk period is shown (f). Nonspecific lysis of T2 cells is indicated by open bars (□). The percentage of tetramer-positive cells is indicated by the num- ber in parentheses for the various days. D, Pro- gressive increase in SL9-tetramerCD8 T cells in culture is shown.

Article Snippet: OptEIA Sets (BD PharMingen) were used to measure the concentrations of IL-2, IL-4, and IFN- in supernatants of CTLs restimulated for 24 h with peptide-pulsed T2 cells (the human TAP-deficient lymphoblast T-B hybridoma) (American Type Culture Collection).

Techniques: Release Assay, Lysis

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The HIV-1 HLA-A2-SLYNTVATL is a help-independent CTL epitope.

doi: 10.4049/jimmunol.172.9.5249

Figure Lengend Snippet: FIGURE 2. Isolation of essentially homogeneous SL9 tetramer-binding T cell lines. A, A SL9-CTL culture containing 57% tetramerCD8 T cells from donor Wsu02 is shown. B and C, Cultures expanded by anti-CD3 mAb and IL-2 from sorted tetramer-binding cells from donors Wsu02 and Wsu03 are shown. D–F, SL9-specific lysis of T2 cells at E:T ratio of 5:1 at four different concentrations of the peptide is shown.

Article Snippet: OptEIA Sets (BD PharMingen) were used to measure the concentrations of IL-2, IL-4, and IFN- in supernatants of CTLs restimulated for 24 h with peptide-pulsed T2 cells (the human TAP-deficient lymphoblast T-B hybridoma) (American Type Culture Collection).

Techniques: Isolation, Binding Assay, Lysis

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The HIV-1 HLA-A2-SLYNTVATL is a help-independent CTL epitope.

doi: 10.4049/jimmunol.172.9.5249

Figure Lengend Snippet: FIGURE 6. Distinctive recognition patterns for 11 natural variants of SL9 by cultures of SL9-CTLs from two donors. A, Shown is the percent cytotoxicity to T2 cells pulsed with SL9 and its variants at the E:T ratio of 10:1 for SL9-CTLs from donors Wsu-01 and Wsu-03. B–D, Shown are the functional avidities of CTL cultures from three donors to SL9 (f—f), 8V (E—E), 6I8V (‚—‚), 3F8V (ƒ—ƒ), and 3F6I8V (—).

Article Snippet: OptEIA Sets (BD PharMingen) were used to measure the concentrations of IL-2, IL-4, and IFN- in supernatants of CTLs restimulated for 24 h with peptide-pulsed T2 cells (the human TAP-deficient lymphoblast T-B hybridoma) (American Type Culture Collection).

Techniques: Functional Assay

Journal: NPJ Science of Food

Article Title: A multiplex microarray lateral flow immunoassay device for simultaneous determination of five mycotoxins in rice

doi: 10.1038/s41538-024-00342-2

Figure Lengend Snippet: Comparison between the performance of microarray lateral flow device (µLFIA) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) in terms of recovery, repeatability, and reproducibility for aflatoxin B 1 (AFB 1 ), T-2 toxin (T2), zearalenone (ZEA), deoxynivalenol (DON), and fumonisin B 1 (FB 1 ) at final concentration of 10 (L1), 20 (L2), and 50 µg/kg (L3)

Article Snippet: Mycotoxin standards—aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2 ), aflatoxin M 1 (AFM 1 ), ochratoxin A (OTA), zearalenone (ZEA), T-2 toxin (T2), HT-2 toxin (HT-2), deoxynivalenol (DON), and fumonisin B 1 (FB 1 ) were purchased from Romer Labs (Biopure, Singapore).

Techniques: Comparison, Microarray, Chromatography, Mass Spectrometry, Concentration Assay, Intra Assay, Inter Assay

Journal: Toxins

Article Title: The Occurrence and Co-Occurrence of Regulated, Emerging, and Masked Mycotoxins in Rice Bran and Maize from Southeast Asia

doi: 10.3390/toxins14080567

Figure Lengend Snippet: Optimised MS/MS parameters for the analysed compounds, including precursor ions, product ions, declustering potential (DP), collision energy (CE), and collision cell exit potential (CXP).

Article Snippet: Mycotoxins standards: aflatoxin B 1 (AFB 1 ), aflatoxin B 2 (AFB 2 ), aflatoxin G 1 (AFG 1 ), aflatoxin G 2 (AFG 2 ), alternariol, beauvericin (BEA), deoxynivalenol (DON), enniatin A (ENN A), enniatin A 1 (ENN A 1 ), enniatin B (ENN B), enniatin B 1 (ENN B 1 ), fumonisin B 1 (FB 1 ), HT-2 toxin (HT-2), ochratoxin A (OTA), T2 toxin (T-2), and zearalenone (ZEN) were obtained from Romer lab, UK.

Techniques:

Journal: Scientific Reports

Article Title: Four translation initiation pathways employed by the leaderless mRNA in eukaryotes

doi: 10.1038/srep37905

Figure Lengend Snippet: ( a ) Schematic representation of the inhibitors action. Note that harringtonine and T-2 toxin are unable to enter elongating ribosomes and pre-associated vacant 80 S particles, while puromycin binds the ribosomes at any stage. ( b ) Translation inhibition by the drugs as revealed by the FLERT technique. Luciferase activities are normalized to those in wells without the inhibitors.

Article Snippet: The following reagents were used in this study: torin1 (Tocris Bioscience), harringtonine (LKT Laboratories), T-2 toxin (Cayman), spermidine trihydrochloride (Sigma-Aldrich), salubrinal and NSC119889 (Santa Cruz Biotechnology).

Techniques: Inhibition, Luciferase